Nernst

__ **Diffusion Potentials Across an Artificial Membrane** __

Membrane properties of Nafion epithelium (the base line for de-focus driven ocular growth)

SETUP AND PREPARATIONS

// CHLORIDING THE ELECTRODE (Ag/Cl) //



// SETUP OF THE SOLUTIONS AND USSING CHAMBER //


 * [[image:IMG0172A.jpg width="288" height="216"]] **

// USSSING CHAMBER //

// SOLUTION FILLED USSING CHAMBER WITH ELECTRODES //

// TOP VIEV OF RECORDING THE POTENTIAL //

// NAFION 324 IN USSING CHAMBER //

= Results so far... (subject to change) =

Asymmetry potential _ 0.6 mV_

** Nafion 115 **

** Table 1 Diffusion Potential of Nafion 115 ** * Average reading minus asymmetry potential
 * **Test number** || || **1 **  ||  **2 **  ||  **3 **  ||  **4 **  ||  **5 **  ||  **6 **  ||  **7 **  ||  **8 **  ||
 * |||||||||||||||| Concentration of NaCl (Molarity)  || ||
 * **Chamber 1 ** || || 0.001  ||  0.001  ||  0.001  ||  0.002  ||  1.0  ||  1.0  ||  1.0  ||  1.0  ||
 * **Chamber 2 ** ||^  ||  0.001  ||  0.01  ||  0.1  ||  1  ||  0.002  ||  0.01  ||  0.1  ||  1  ||
 * **Concentration Difference ** ||^  ||  1 X  ||  10 X  ||  100 X  ||  500 X  ||  500 X  ||  100 X  ||  10 X  ||  1 X  ||
 * **Diffusion Potential reading(mV) ** || a.  ||  0  ||  -111.3  || ||  -125.0  ||  114  ||  85.4  ||  2.5  ||  0  ||
 * || b.  ||  0  ||  -108.5  || ||  -123.5  ||  113.6  ||  86.5  ||  2.4  ||  0  ||
 * ^  ||  c.  ||  0  ||  -105.2  || ||  -123.2  ||  113.3  ||  66.4  ||  2.3  ||  0  ||
 * **Corrected Average reading*(mV) ** || || || ** -108.93 **  || ||  ** -124.5 **  ||  ** 113.03 **  ||  ** 78.83 **  ||  ** 1.8 **  ||  ** 0 **  ||

** N **** afion 324 **

** Table 2 Diffusion Potential of Nafion 324 ** || || ** -104.8 **  ||  ** 113.5 **  ||  ** 74.867 **  ||  ** 1.37 **  ||  ** 0 **  || * Average reading minus asymmetry potential
 * **Test number** ||  || **1 ** || **<span style="font-family: 'Cambria','serif';">2 ** || **<span style="font-family: 'Cambria','serif';">3 ** || **<span style="font-family: 'Cambria','serif';">4 ** || **<span style="font-family: 'Cambria','serif';">5 ** || **<span style="font-family: 'Cambria','serif';">6 ** || **<span style="font-family: 'Cambria','serif';">7 ** || **<span style="font-family: 'Cambria','serif';">8 ** ||
 * |||||||||||||||| Concentration of NaCl (Molarity) ||  ||
 * **<span style="font-family: 'Cambria','serif';">Chamber 1 ** ||  || 0.001 || 0.001 || 0.001 || 0.002 || 1.0 || 1.0 || 1.0 || 1.0 ||
 * **<span style="font-family: 'Cambria','serif';">Chamber 2 ** ||^  || 0.001 || 0.01 || 0.1 || 1 || 0.002 || 0.01 || 0.1 || 1 ||
 * **<span style="font-family: 'Cambria','serif';">Concentration Difference ** ||^  || 1 X || 10 X || 100 X || 500 X || 500 X || 100 X || 10 X || 1 X ||
 * **<span style="font-family: 'Cambria','serif';">Diffusion Potential reading (mV) ** || a. || 0 || -26.6 ||  || -104.4 || 115 || 75.4 || 2.4 || 0 ||
 * || b. || 0 || -27.5 ||  || -104. || 114.1 || 75.6 || 1.7 || 0 ||
 * || c. || 0 || -28.6 ||  || -104.1 || 113.2 || 75.4 || 1.8 || 0 ||
 * **<span style="font-family: 'Cambria','serif';">Corrected Average reading* (mV) ** ||  ||   ||  ** -28.17 **